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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Development of Immunological Test Kit for the Detection of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in Swine) ผู้เขียน: สุรศักดิ์ จิตตวิสุทธิกุล,  นางอมรา ทองปาน, รองศาสตราจารย์ , Orawan Boodde, Wilairat Shumsing, วรวิทย์ วัชชวัลคุ สื่อสิ่งพิมพ์:pdf AbstractTwo types of porcine reproductive and respiratory syndrome virus (PRRSV), European (EU)
and North American (US), cause an important swine disease called porcine reproductive and respiratory
syndrome (PRRS). An enzyme-linked immunosorbent assay (ELISA) for the simultaneous detection of
serum antibodies against these two PRRS was developed. A fused protein containing EU PRRSV and
US PRRSV nucleocapsid proteins (USEU-rN protein) expressed in Escherichia coli were partial purified
and used as antigens.
As determined by a checkerboard titration, an optimal dilution of the USEU-rN protein and
serum were 1:1600 and 1:40, respectively. The optimal cut-off value for the developed USEU PRRS
ELISA was 0.4, with diagnostic sensitivity and specificity of 97.5% and 100%, respectively. Using
Two Graph-ROC program testing with 200 positive sera (IDEXX? HerdCheck PRRS ELISA) and with
200 negative sera from PRRS-free country (Denmark), comparison of 1,077 field sera obtained from
USEU PRRS ELISA with those from IDEXX? ELISA showed the degree of agreement (k value) was
highly obtained at 0.7916. The kit is considered reliable for routine diagnosis, epidemiological surveys
and outbreak investigations. |
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 Researcherนาง วิไลรัตน์ ฉ่ำสิงห์ที่ทำงาน:ภาควิชาเวชศาสตร์และทรัพยากรการผลิตสัตว์ คณะสัตวแพทยศาสตร์ สาขาที่สนใจ: Animal Karyotyping, Cell culture and Viral Isolation Resume |
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